Lady Ana was a member of the Forward Order in the Caligo Forest on Dominaria. She was the mentor of Liliana Vess.
One time, when Liliana's brother Josu suffered a horrible corruption at the hands of their father's enemies, Ana tasked Liliana to gather the roots of the Esis Tree for his cure. Unfortunately, she found out that her father's dark enemies had razed the groves where the Esis Tree had grown. She only learned of this fact when she encountered a strange man who claimed to be a supporter of her father. He offered his assistance, though he warned that her family would not want her to use his cure.
Liliana returned to the castle and despite the warnings of Lady Ana, she used the cure. Truly, it cured Josu of his affliction, but only at the cost of driving him insane and poisoning him anew. Several of the servants, Ana among them, were killed by Josu. Realizing that she had to kill the crazed creature that Josu had become, Liliana turned his victims into zombies to fight him. At that same moment, she ignited her planeswalker's spark and ended up on Innistrad.